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All these touchy jobs are properly done by technology. Nowadays with really good accounting application and word processing tools, it is fair to say that technologies have greater accuracy and decreased matlab programming margin of inaccuracy. Whereas for data entry, some accountants still use Microsoft Excel and working ledgers, many companies will purchase special program that adds simplified data entry and correct ledgers ends up in more accurate economic reports. This is advantageous for agencies as a result of inaccuracy can result in tax consequences or uninformed determination makers Logica. ugent. be 2018. We constructed matlab simple tool using Matlab to automate matlab programming colocalization procedure and to exclude matlab programming biased estimations resulting from visual inspections of images. The script in Matlab language code instantly imports confocal images and converts them into arrays. The contrast of all images is uniformly set by linearly reassigning matlab programming values of pixel intensities to use matlab programming full 8 bit range 0 255. Images are binarized on a number of threshold levels. The area above matlab sure threshold level is summed for each channel of matlab programming image and for colocalized areas. As matlab result, count of pixels above a couple of threshold levels in any number of images is saved in an ASCII file.